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Peptide Calculator

Peptide Concentration & Volume Calculator
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Peptide Concentration & Volume Calculator

Compute concentration (mg/mL), required volume (mL), and U‑100 unit‑scale equivalents for research preparations. Includes forward calculations, reverse calculations, and multi‑peptide blend ratio calculations.

Mass to Units Calculator

Converts a specified mass-per-aliquot target into U‑100 unit‑scale equivalents.

Target Mass per Aliquot
Vial Content (mg)
Total peptide content per vial
Diluent Volume (mL)
Diluent volume for reconstitution
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Enter values to generate calculated outputs

Diluent Types for Reconstitution

A practical breakdown of common diluent types referenced for peptide reconstitution.

Bacteriostatic Water (BAC)

Sterile water with a preservative (commonly benzyl alcohol) designed for multi‑access preparations. Helps slow bacterial growth after the vial has been punctured multiple times.

Common context
  • Multi‑target mass vials accessed repeatedly
  • Convenience + reduced contamination risk

Sterile Water

Sterile water without preservative. Meant for single‑access situations. Once opened or punctured, it has no preservative help against contamination.

Common context
  • Single‑access preparation where the solution won’t be stored long
  • Cases where preservatives are specifically avoided

Non‑sterile water

Not sterile. Even “clean” tap water can contain microorganisms, minerals, and impurities that can ruin the solution and increase contamination risk.

Avoid in research prep
  • Risk of contamination/infection
  • Unpredictable minerals/chemicals that can destabilize peptides
General rule: for multi‑access vials, bacteriostatic water is commonly preferred. Sterile water may be selected for single‑access preparation scenarios, depending on protocol requirements. Non‑sterile water is not recommended for reconstitution due to contamination risk.

Quick Guide (Research Math)

Plain‑language definitions of inputs and outputs (research math only).

1

Mass Input

Specify the target mass amount. Many peptide values are expressed in micrograms (mcg), while higher values may be expressed in milligrams (mg). The unit toggle changes the input unit.

2

Total Mass in Vial

Represents total peptide powder content in the vial, typically labeled in milligrams (mg). Common strengths include 5 mg, 10 mg, and 15 mg.

3

Diluent Volume (mL)

Represents the bacteriostatic water (BAC water) volume applied for reconstitution. Higher volumes produce lower concentrations; lower volumes produce higher concentrations.

4

Outputs (What the calculator reports)

Outputs (What the calculator reports) include concentration (mg/mL), volume (mL), unit‑scale equivalents (assuming 100 units = 1 mL), and an estimated target mass count per vial.

Worked Calculation Example

Scenario: A 5 mg vial is reconstituted with 3 mL bacteriostatic water and evaluated at a 250 mcg target mass per aliquot.
→ Concentration:
5 mg ÷ 3 mL = 1.67 mg/mL
→ Mass in mg:
250 mcg = 0.25 mg
→ Volume per aliquot:
0.25 mg ÷ 1.67 mg/mL = 0.15 mL
→ U‑100 unit scale:
0.15 mL × 100 = 15 units
→ Mass per vial:
5 mg ÷ 0.25 mg = 20 aliquots
Result: The calculated unit‑scale equivalent is 15 units (U‑100) per 250 mcg target mass per aliquot, yielding an estimated 20 aliquots per 5 mg vial.
Important: Educational and research reference only. Verify calculations independently. Unit conversions assume a standard U‑100 reference scale (100 units = 1 mL).

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